حوزه دانش :میوه های هسته دار
محل انتشار مقاله :همایش / کنگره خارجی
نام نشریه :4th international symposium on Acclimatization & Establishment of Micropropagated plants. 8th - 12th december 2008 Bangalor
A micropropagation approach was developed for PHILA dwarfing cherry rootstock which was produced by crossing Prunus avium and Prunus mahaleb. The micropropagation cycle includes establishment, shoot proliferation, root Induction and plant acclimatization steps that were examined using various media and growth regulators. Shoot tips and axillary buds, derived from greenhouse-grown one-year-old trces, used as explants and were successfully established in vitro. Multiplication rate of about 5.37 was achieved over a four week period using MS medium with 0.5 mg/l benzyladenine. High rooting responses, with up to 100 percent efficiency, were also obtained within four weeks on DKW medium without growth regulators. Moreover, high rooting responses were obtained using MS plus 0.5 mg/I IBA and MS without growth regulators mediums with 85 and 75 percent efliciency, respectively. Rooted plantlets were transferred to peat moss and coco peat mixture (1:1 v/v) that were fertilized with inorganic salts. Acclimatization was affected directly by rooting conditions. Survival was best when plantlets were transferred to pots and had high quality of rooting.